pLVX-puro_G-CEPIA1er
(Plasmid #164590)

• Purpose: Stable integration and expression of G-CEPIA1er in mammalian cells
• Depositing Lab: Joseph Hyser
• Publication: Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10.1038/s41598-019-46856-8.

Backbone

• Vector backbone: pLVX-puro
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 8037
• Total vector size (bp): 9535
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G-CEPIA1er
• Species: Synthetic
• Insert Size (bp): 1497
• Mutation: G-GECO1.1 E299D M304L D328N N345K F360W G369S E372D V395E D401E
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • pLVX-puro_G-CEPIA1er.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry