pLVX-puro_GCaMP6s
(Plasmid #164589)

• Purpose: Stable integration and expression of GCaMP6s in mammalian cells
• Depositing Lab: Joseph Hyser
• Publication: Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10.1038/s41598-019-46856-8.

Backbone

• Vector backbone: pLVX-puro
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 8071
• Total vector size (bp): 9514
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GCaMP6s
• Species: Synthetic
• Insert Size (bp): 1443
• Promoter: CMV
• Tags / Fusion Proteins:
  • 6xHis (N terminal on insert)
  • T7 tag (N terminal on insert)
  • Xpress tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: - (unknown if destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: None

Resource Information

• Supplemental Documents:
  • Depositor's Reference Sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry