SARS-CoV-2 Spike-d18
(Plasmid #164583)

• Purpose: A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.
• Depositing Lab: Jason Sheltzer
• Publication: SARS-CoV-2 Spike plasmid to generate pseudovirus (unpublished)

Backbone

• Vector backbone: pRP
• Backbone manufacturer: Vectorbuilder
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 Spike
• Species: SARS-CoV-2
• Insert Size (bp): 3768
• Mutation: Deleted amino acids 1256-1273
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: EF1a

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTGTCGTGACAAGTTTGTAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: SinoBiological Cat No. VG40589-UT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Codon-optimized SARS-CoV-2 Spike was cloned from SinoBiological plasmid VG40589-UT. An 18-amino acid truncation was introduced to improve expression. The plasmid can be used to generate Spike-pseudotyped lentiviral or VSV particles.