pUpstream2Lox
(Plasmid #164573)

• Purpose: Integration of 2 LoxN sites in the genome of P. berghei
• Depositing Lab: Olivier Silvie
• Publication: Silvie lab unpublished (unpublished)

Backbone

• Vector backbone: pBluescript
• Vector type: Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR)
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1350
• Entrez Gene: DHFR (a.k.a. DHFR1, DHFRP1, DYR)
• Promoter: PyHSP70 promoter (PY17X_0712100)

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: bifunctional yeast enzyme cytosine deaminase and uridyl phosphoribosyl transferase (yFCU)
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1107
• Entrez Gene: FCY1 (a.k.a. YPR062W)
• Entrez Gene: FUR1 (a.k.a. YHR128W)
• Promoter: Bidirectional PbEF1alpha promoter (PBANKA_1133300)

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pUpstream2Lox.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry