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Addgene

pαH-S-GSAS
(Plasmid #164565)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164565 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pαH
  • Backbone size w/o insert (bp) 4746
  • Total vector size (bp) 8370
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spike (S-GSAS variant)
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    3624
  • Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RRAR) replaced with GSAS
  • GenBank ID
    MN908947
  • Tags / Fusion Proteins
    • HRV3C cleavage site (before tags) (C terminal on backbone)
    • 8X His tag (C terminal on backbone)
    • 2X Strep-Tag II (C terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jason McLellan

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pαH-S-GSAS was a gift from Priyamvada Acharya (Addgene plasmid # 164565 ; http://n2t.net/addgene:164565 ; RRID:Addgene_164565)

  • For your References section:

    D614G Mutation Alters SARS-CoV-2 Spike Conformation and Enhances Protease Cleavage at the S1/S2 Junction. Gobeil SM, Janowska K, McDowell S, Mansouri K, Parks R, Manne K, Stalls V, Kopp MF, Henderson R, Edwards RJ, Haynes BF, Acharya P. Cell Rep. 2021 Jan 12;34(2):108630. doi: 10.1016/j.celrep.2020.108630. Epub 2020 Dec 26. 10.1016/j.celrep.2020.108630 PubMed 33417835
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