SaLgCG
(Plasmid
#164563)
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Purpose(Empty Backbone) GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164563 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSaLgCP
- Backbone size (bp) 12239
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Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter U6 promoter for crRNA expression and EFS promoter for GFP expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsS2C mutation in AmpR may result in low growth.
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SaLgCG was a gift from Junwei Shi (Addgene plasmid # 164563 ; http://n2t.net/addgene:164563 ; RRID:Addgene_164563) -
For your References section:
ALC1 links chromatin accessibility to PARP inhibitor response in homologous recombination-deficient cells. Verma P, Zhou Y, Cao Z, Deraska PV, Deb M, Arai E, Li W, Shao Y, Puentes L, Li Y, Patankar S, Mach RH, Faryabi RB, Shi J, Greenberg RA. Nat Cell Biol. 2021 Jan 18. pii: 10.1038/s41556-020-00624-3. doi: 10.1038/s41556-020-00624-3. 10.1038/s41556-020-00624-3 PubMed 33462394