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Addgene

SaLgCP
(Plasmid #164562)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164562 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SaLgCP
  • Backbone size (bp) 12110
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Promoter U6 promoter for crRNA expression and EFS promoter for Puro expression
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • Flag-NLS-SaCas9-P2A-PuroR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SaLgCP was a gift from Junwei Shi (Addgene plasmid # 164562 ; http://n2t.net/addgene:164562 ; RRID:Addgene_164562)

  • For your References section:

    ALC1 links chromatin accessibility to PARP inhibitor response in homologous recombination-deficient cells. Verma P, Zhou Y, Cao Z, Deraska PV, Deb M, Arai E, Li W, Shao Y, Puentes L, Li Y, Patankar S, Mach RH, Faryabi RB, Shi J, Greenberg RA. Nat Cell Biol. 2021 Jan 18. pii: 10.1038/s41556-020-00624-3. doi: 10.1038/s41556-020-00624-3. 10.1038/s41556-020-00624-3 PubMed 33462394
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