PD29
(Plasmid
#164542)
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PurposeExpresses Drd1-FLAG
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 164542 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePhage lentivirus
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Vector typeLentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDopamine receptor D1
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SpeciesM. musculus (mouse)
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MutationWT
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Entrez GeneDrd1 (a.k.a. C030036C15Rik, Drd-1, Drd1a, Gpcr15)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PD29 was a gift from Gregory Pazour (Addgene plasmid # 164542 ; http://n2t.net/addgene:164542 ; RRID:Addgene_164542) -
For your References section:
Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling. Desai PB, Stuck MW, Lv B, Pazour GJ. J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 10.1083/jcb.201912104. 10.1083/jcb.201912104 PubMed 32435793