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Addgene

PD19
(Plasmid #164528)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164528 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Phage lentivirus
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin noK
  • Species
    M. musculus (mouse)
  • Mutation
    All seven Lysines of Ubiquitin changed to Arginine
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • Ub noK (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PD19 was a gift from Gregory Pazour (Addgene plasmid # 164528 ; http://n2t.net/addgene:164528 ; RRID:Addgene_164528)

  • For your References section:

    Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling. Desai PB, Stuck MW, Lv B, Pazour GJ. J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 10.1083/jcb.201912104. 10.1083/jcb.201912104 PubMed 32435793
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