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Addgene

pAN68
(Plasmid #164525)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164525 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-Blast
  • Backbone size w/o insert (bp) 5879
  • Vector type
    Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CENP-R uORF-1
  • Alt name
    ITGB3BP altORF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    114
  • Entrez Gene
    ITGB3BP (a.k.a. CENP-R, CENPR, HSU37139, NRIF3, TAP20)
  • Tag / Fusion Protein
    • -6xhis-precission-GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAN68 was a gift from Iain Cheeseman (Addgene plasmid # 164525 ; http://n2t.net/addgene:164525 ; RRID:Addgene_164525)
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