pAN289
(Plasmid
#164524)
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PurposeRetroviral expression of CENP-R uORF-1
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164524 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIC242
- Backbone size w/o insert (bp) 6672
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Vector typeRetroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCENP-R uORF-1
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Alt nameITGB3BP altORF1
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SpeciesH. sapiens (human)
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Insert Size (bp)114
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Entrez GeneITGB3BP (a.k.a. CENP-R, CENPR, HSU37139, NRIF3, TAP20)
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Tag
/ Fusion Protein
- eGFP-Tev-S (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site SacII (not destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAN289 was a gift from Iain Cheeseman (Addgene plasmid # 164524 ; http://n2t.net/addgene:164524 ; RRID:Addgene_164524)