pDONR207 SARS-CoV-2 NSP1 124A/125A
(Plasmid
#164523)
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PurposeGateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions R124A/K125A)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164523 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR207
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNSP1 124A/125A
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SpeciesOther
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MutationInsert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 with amino acid substitutions R124A/K125A.
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR207 SARS-CoV-2 NSP1 124A/125A was a gift from Mitchell Guttman (Addgene plasmid # 164523 ; http://n2t.net/addgene:164523 ; RRID:Addgene_164523) -
For your References section:
SARS-CoV-2 Disrupts Splicing, Translation, and Protein Trafficking to Suppress Host Defenses. Banerjee AK, Blanco MR, Bruce EA, Honson DD, Chen LM, Chow A, Bhat P, Ollikainen N, Quinodoz SA, Loney C, Thai J, Miller ZD, Lin AE, Schmidt MM, Stewart DG, Goldfarb D, De Lorenzo G, Rihn SJ, Voorhees RM, Botten JW, Majumdar D, Guttman M. Cell. 2020 Nov 25;183(5):1325-1339.e21. doi: 10.1016/j.cell.2020.10.004. Epub 2020 Oct 8. 10.1016/j.cell.2020.10.004 PubMed 33080218