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Addgene

pDONR207 SARS-CoV-2 NSP1 Delta RC
(Plasmid #164522)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164522 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR207
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NSP1 Delta RC
  • Species
    Other
  • Mutation
    Insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 with amino acid substitutions K164A/H165A.
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR207 SARS-CoV-2 NSP1 Delta RC was a gift from Mitchell Guttman (Addgene plasmid # 164522 ; http://n2t.net/addgene:164522 ; RRID:Addgene_164522)

  • For your References section:

    SARS-CoV-2 Disrupts Splicing, Translation, and Protein Trafficking to Suppress Host Defenses. Banerjee AK, Blanco MR, Bruce EA, Honson DD, Chen LM, Chow A, Bhat P, Ollikainen N, Quinodoz SA, Loney C, Thai J, Miller ZD, Lin AE, Schmidt MM, Stewart DG, Goldfarb D, De Lorenzo G, Rihn SJ, Voorhees RM, Botten JW, Majumdar D, Guttman M. Cell. 2020 Nov 25;183(5):1325-1339.e21. doi: 10.1016/j.cell.2020.10.004. Epub 2020 Oct 8. 10.1016/j.cell.2020.10.004 PubMed 33080218
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