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Addgene

pB-6HIS-TEV-mEGFP,IRES-NGFR
(Plasmid #164521)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164521 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-TAG-ERN
  • Modifications to backbone
    GFP sequence contains an amino acid substitution (A205K) to generate a monomeric variant based on previous literature (Alberti et al).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-6HIS-TEV-mEGFP,IRES-NGFR was a gift from Mitchell Guttman (Addgene plasmid # 164521 ; http://n2t.net/addgene:164521 ; RRID:Addgene_164521)

  • For your References section:

    SARS-CoV-2 Disrupts Splicing, Translation, and Protein Trafficking to Suppress Host Defenses. Banerjee AK, Blanco MR, Bruce EA, Honson DD, Chen LM, Chow A, Bhat P, Ollikainen N, Quinodoz SA, Loney C, Thai J, Miller ZD, Lin AE, Schmidt MM, Stewart DG, Goldfarb D, De Lorenzo G, Rihn SJ, Voorhees RM, Botten JW, Majumdar D, Guttman M. Cell. 2020 Nov 25;183(5):1325-1339.e21. doi: 10.1016/j.cell.2020.10.004. Epub 2020 Oct 8. 10.1016/j.cell.2020.10.004 PubMed 33080218
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