WWP-Luc (p21/WAF1 promoter)
(Plasmid #16451)

• Depositing Lab: Bert Vogelstein
• Publication: el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Backbone

• Vector backbone: pBluescript II KS (+) + luc
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: WAF1 promoter
• Alt name: p21
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• Entrez Gene: CDKN1A (a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 36 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 2.4kb WAF1 promoter region was obtained by PCR amplification using a P1-WAF1 subclone as template and the primers 5’-CCACAAGCTTCTGACTTCGGCAG-3’and 5’CCCAGGAACAAGCTTGGGCAGCAG-3’. This PCR fragment was cloned into the HindIII site of pBluescript KS(+) (Stratagene) to yield plasmid pWWP. WWP-Luc was cloned by inserting the 2.6kb BamHl luciferase cassette from PG13-Luc into the Xhol sites of pWWP.

Please note that there are a few discrepancies between Addgene's quality control sequence and the top GenBank hit for the promoter sequence. The plasmid should function as reported.