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pETM10 with rat Δ192Annexin A11
(Plasmid #164498)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164498 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Backbone manufacturer
    Gunter Stier
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    rat Δ192Annexin A11
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    900
  • Mutation
    N-terminal truncation
  • Entrez Gene
    Anxa11
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site Acc65I (not destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The cDNAs were obtained by us by using PC12 cells, RT-PCR and specific primers. The cDNAs are inserted in the pETM10 vector between the NcoI and Acc65I (Kpn) sites. The vector, pETM10, is an EMBL vector, which was a generous gift from its maker, Gunter Stier.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETM10 with rat Δ192Annexin A11 was a gift from Anni Vedeler (Addgene plasmid # 164498 ; http://n2t.net/addgene:164498 ; RRID:Addgene_164498)

  • For your References section:

    Structure of the ALS Mutation Target Annexin A11 Reveals a Stabilising N-Terminal Segment. Lillebostad PAG, Raasakka A, Hjellbrekke SJ, Patil S, Rostbo T, Hollas H, Sakya SA, Szigetvari PD, Vedeler A, Kursula P. Biomolecules. 2020 Apr 24;10(4). pii: biom10040660. doi: 10.3390/biom10040660. 10.3390/biom10040660 PubMed 32344647
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