CMV::TVA/tRFP
(Plasmid #164486)

• Purpose: Dual CMV promoter plasmid to express TVA and TurboRFP
• Depositing Lab: Hollis Cline
• Publication: Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2021. doi: 10.1523/ENEURO.0477-20.2021.

Backbone

• Vector backbone: biCS2 (bicistronic pCS2)
• Backbone size w/o insert (bp): 4500
• Total vector size (bp): 5639
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: TVA
• Species: G. gallus (chicken)
• Insert Size (bp): 363
• Entrez Gene: CD320 (a.k.a. TVA)
• Promoter: sCMV IE94

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: SP6

Gene/Insert 2

• Gene/Insert name: TurboRFP
• Alt name: tRFP
• Species: Entacmaea quadricolor
• Insert Size (bp): 706
• Promoter: sCMV

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: M13 Reverse

Resource Information

• Supplemental Documents:
  • CMV-TVA-tRFP.gbk
• A portion of this plasmid was derived from a plasmid made by: Edward Callaway, Salk Institute

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry