CMV::TVA/tRFP
(Plasmid
#164486)
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PurposeDual CMV promoter plasmid to express TVA and TurboRFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonebiCS2 (bicistronic pCS2)
- Backbone size w/o insert (bp) 4500
- Total vector size (bp) 5639
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameTVA
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SpeciesG. gallus (chicken)
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Insert Size (bp)363
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Entrez GeneCD320 (a.k.a. TVA)
- Promoter sCMV IE94
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameTurboRFP
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Alt nametRFP
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SpeciesEntacmaea quadricolor
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Insert Size (bp)706
- Promoter sCMV
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer M13 Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byEdward Callaway, Salk Institute
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV::TVA/tRFP was a gift from Hollis Cline (Addgene plasmid # 164486 ; http://n2t.net/addgene:164486 ; RRID:Addgene_164486) -
For your References section:
Application of Recombinant Rabies Virus to Xenopus Tadpole Brain. Faulkner RL, Wall NR, Callaway EM, Cline HT. eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2021. doi: 10.1523/ENEURO.0477-20.2021. 10.1523/ENEURO.0477-20.2021 PubMed 34099488