pLenti sfGFP-LAMP1-mCherry (pHLARE)
(Plasmid #164478)

• Purpose: Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.
• Depositing Lab: Diane Barber
• Publication: Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383. doi: 10.1091/mbc.E20-06-0383.

Backbone

• Vector backbone: pLenti
• Backbone size w/o insert (bp): 6900
• Total vector size (bp): 9600
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LAMP1
• Species: R. norvegicus (rat)
• Insert Size (bp): 2755
• Entrez Gene: Lamp1 (a.k.a. LGP120)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mCherry (C terminal on insert)
  • superfolder GFP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F, GFP-F, mCherry-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note there is a single mutation in sfGFP-D117Y. Depositor confirms this is not a concern for plasmid function.