pOX-twk16
(Plasmid
#16444)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16444 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOX
- Backbone size w/o insert (bp) 3200
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Vector typeXenopus Oocyte expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameC.elegans Tandem pore domain K+ channel twk-16
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Alt nameF52E4.4
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SpeciesC. elegans (nematode)
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Insert Size (bp)1650
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GenBank IDNM_076125
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Entrez Genetwk-16
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOX-twk16 was a gift from Larry Salkoff (Addgene plasmid # 16444 ; http://n2t.net/addgene:16444 ; RRID:Addgene_16444) -
For your References section:
Evolution tunes the excitability of individual neurons. Salkoff L, Butler A, Fawcett G, Kunkel M, McArdle C, Paz-y-Mino G, Nonet M, Walton N, Wang ZW, Yuan A, Wei A. Neuroscience. 2001 . 103(4):853-9. 10.1016/S0306-4522(01)00079-3 PubMed 11301195