AAV-CMV-H2B-FusionRed
(Plasmid #164430)

• Purpose: AAV plasmid expressing nuclear-localized FusionRed fluorophore
• Depositing Lab: Connie Cepko
• Publication: Wang et al Mol Ther Methods Clin Dev. 2020 Oct 10;19:376-386. doi: 10.1016/j.omtm.2020.10.003. eCollection 2020 Dec 11.

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• Serotype: Select serotype for details See details about
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Backbone

• Vector backbone: AAV-MCS8
• Backbone manufacturer: Jeng-Shin Lee, Harvard University
• Backbone size w/o insert (bp): 6183
• Total vector size (bp): 7278
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: H2B-FusionRed
• Species: Synthetic
• Insert Size (bp): 1095
• Promoter: CMV
• Tag / Fusion Protein:
  • H2B

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A (whole plasmid sequencing)

Resource Information

• Supplemental Documents:
  • AAV-CMV-H2B-FusionRed.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry