AAV-CMV-H2B-FusionRed
(Plasmid
#164430)
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PurposeAAV plasmid expressing nuclear-localized FusionRed fluorophore
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164430 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneAAV-MCS8
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Backbone manufacturerJeng-Shin Lee, Harvard University
- Backbone size w/o insert (bp) 6183
- Total vector size (bp) 7278
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameH2B-FusionRed
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SpeciesSynthetic
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Insert Size (bp)1095
- Promoter CMV
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Tag
/ Fusion Protein
- H2B
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A (whole plasmid sequencing) (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-CMV-H2B-FusionRed was a gift from Connie Cepko (Addgene plasmid # 164430 ; http://n2t.net/addgene:164430 ; RRID:Addgene_164430) -
For your References section:
In Situ Detection of Adeno-associated Viral Vector Genomes with SABER-FISH. Wang SK, Lapan SW, Hong CM, Krause TB, Cepko CL. Mol Ther Methods Clin Dev. 2020 Oct 10;19:376-386. doi: 10.1016/j.omtm.2020.10.003. eCollection 2020 Dec 11. 10.1016/j.omtm.2020.10.003 PubMed 33209963
