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AAV-hRedOpsin-TGFB1
(Plasmid #164427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV-MCS8
  • Backbone manufacturer
    Jeng-Shin Lee, Harvard University
  • Backbone size w/o insert (bp) 6062
  • Total vector size (bp) 7244
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tgfb1
  • Alt name
    transforming growth factor beta 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1182
  • Entrez Gene
    Tgfb1 (a.k.a. TGF-beta1, TGFbeta1, Tgfb, Tgfb-1)
  • Promoter human red opsin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer gtggccagatcctgtccaaa
  • 3′ sequencing primer tccgctggattgagggccgaa
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-hRedOpsin-TGFB1 was a gift from Connie Cepko (Addgene plasmid # 164427 ; http://n2t.net/addgene:164427 ; RRID:Addgene_164427)

  • For your References section:

    Microglia modulation by TGF-beta1 protects cones in mouse models of retinal degeneration. Wang SK, Xue Y, Cepko CL. J Clin Invest. 2020 Aug 3;130(8):4360-4369. doi: 10.1172/JCI136160. 10.1172/JCI136160 PubMed 32352930
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