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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16441 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV-Neo-Bam
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Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 6600
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMDM2
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SpeciesH. sapiens (human)
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Entrez GeneMDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full-length human MDM2 cDNA.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV MDM-2 was a gift from Bert Vogelstein (Addgene plasmid # 16441 ; http://n2t.net/addgene:16441 ; RRID:Addgene_16441) -
For your References section:
Amplification of a gene encoding a p53-associated protein in human sarcomas. Oliner JD, Kinzler KW, Meltzer PS, George DL, Vogelstein B. Nature. 1992 Jul 2. 358(6381):80-3. 10.1038/358080a0 PubMed 1614537
Map uploaded by the depositor.
