pCMV-Neo-Bam
(Plasmid #16440)

• Purpose: (Empty Backbone) Contains two independent transcription units--a CMV promoter/enhancer upstream of a MCS and a HSV thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.
• Depositing Lab: Bert Vogelstein
• Publication: Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Backbone

• Vector backbone: pCMV-Neo-Bam
• Backbone manufacturer: Vogelstein Lab
• Backbone size (bp): 6600
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: Bglob-intron-F
• 3′ sequencing primer: Bglob-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The vector pCMV-Neo-Bam was engineered to contain two independent transcription units. The first unit is comprised of a CMV promoter/enhancer upstream of a multiple cloning site, and splice and polyA sites to ensure appropriate processing. The second transcription unit includes a herpes simplex virus (HSV) thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.