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pCMV-Neo-Bam p53 R175H
(Plasmid #16436)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16436 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Neo-Bam
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p53 R175H
  • Alt name
    p53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Mutation
    R175H
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Bglob-intron-F
  • 3′ sequencing primer Bglob-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that there are multiple EcoRI sites in this plasmid besides those used for cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Neo-Bam p53 R175H was a gift from Bert Vogelstein (Addgene plasmid # 16436 ; http://n2t.net/addgene:16436 ; RRID:Addgene_16436)

  • For your References section:

    Suppression of human colorectal carcinoma cell growth by wild-type p53. Baker SJ, Markowitz S, Fearon ER, Willson JK, Vogelstein B. Science. 1990 Aug 24. 249(4971):912-5. 10.1126/science.2144057 PubMed 2144057
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