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pCMV-Neo-Bam p53 V143A
(Plasmid #16435)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16435 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Neo-Bam
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p53 V143A
  • Alt name
    p53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Mutation
    V143A. Mutant cDNA from human colorectal tumor CX3.
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A 1.8kb XbaI fragment encoding mutant p53 (from nucleotide -130 to 1671 relative to the ATG) was blunt-ended and ligated to BamHI linkers. This was then cloned into the unique BamHI site in the pCMV-Neo-Bam vector. This plasmid is also known as pC53-SCX3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Neo-Bam p53 V143A was a gift from Bert Vogelstein (Addgene plasmid # 16435 ; http://n2t.net/addgene:16435 ; RRID:Addgene_16435)

  • For your References section:

    Suppression of human colorectal carcinoma cell growth by wild-type p53. Baker SJ, Markowitz S, Fearon ER, Willson JK, Vogelstein B. Science. 1990 Aug 24. 249(4971):912-5. 10.1126/science.2144057 PubMed 2144057
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