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Addgene

pOX-twk18
(Plasmid #16430)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16430 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOX
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Xenopus Oocyte expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tandem pore domain K+ channel twk-18
  • Alt name
    mah-2, unc-110
  • Alt name
    C24A3.6
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1400
  • Mutation
    N/A
  • GenBank ID
    NM_077115
  • Entrez Gene
    twk-18 (a.k.a. CELE_C24A3.6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpen1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOX-twk18 was a gift from Larry Salkoff (Addgene plasmid # 16430 ; http://n2t.net/addgene:16430 ; RRID:Addgene_16430)

  • For your References section:

    Mutants of a temperature-sensitive two-P domain potassium channel. Kunkel MT, Johnstone DB, Thomas JH, Salkoff L. J Neurosci. 2000 Oct 15. 20(20):7517-24. PubMed 11027209
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