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Addgene

pAGNT-Sema3A
(Plasmid #16428)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16428 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAG-NT
  • Backbone size w/o insert (bp) 6000

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sema3A
  • Alt name
    Semaphorin 3A
  • Alt name
    collapsin 1
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    2200
  • Entrez Gene
    SEMA3A (a.k.a. COLL-1, semaphorin-3A)
  • Tags / Fusion Proteins
    • Myc (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jonathan A. Raper, University of Pennsylvania

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAGNT-Sema3A was a gift from Jonathan Raper (Addgene plasmid # 16428 ; http://n2t.net/addgene:16428 ; RRID:Addgene_16428)
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