pAGNT-Sema3A
(Plasmid
#16428)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16428 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepAG-NT
- Backbone size w/o insert (bp) 6000
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSema3A
-
Alt nameSemaphorin 3A
-
Alt namecollapsin 1
-
SpeciesG. gallus (chicken)
-
Insert Size (bp)2200
-
Entrez GeneSEMA3A (a.k.a. COLL-1, semaphorin-3A)
-
Tags
/ Fusion Proteins
- Myc (N terminal on backbone)
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byJonathan A. Raper, University of Pennsylvania
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAGNT-Sema3A was a gift from Jonathan Raper (Addgene plasmid # 16428 ; http://n2t.net/addgene:16428 ; RRID:Addgene_16428)