Nup54 no FG-mEGFP494(0)
(Plasmid
#164269)
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PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164269 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneunspecified
- Total vector size (bp) 5967
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNup54
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SpeciesH. sapiens (human)
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Mutationdeleted the FG region of Nup54
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GenBank ID53371
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Entrez GeneNUP54
- Promoter CMV
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Tag
/ Fusion Protein
- mEGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Nup54 no FG-mEGFP494(0) was a gift from Sanford Simon (Addgene plasmid # 164269 ; http://n2t.net/addgene:164269 ; RRID:Addgene_164269) -
For your References section:
Conformation of the nuclear pore in living cells is modulated by transport state. Pulupa J, Prior H, Johnson DS, Simon SM. Elife. 2020 Dec 21;9. pii: 60654. doi: 10.7554/eLife.60654. 10.7554/eLife.60654 PubMed 33346731
