pMTP112 (pMS26_miniTn6900(KanR))
(Plasmid #164260)

• Purpose: Delivery vector to insert the miniTn6900(KanR) element into the E. coli chromosome at the neutral Tn7 attachment site inside a miniTn7 element.
• Depositing Lab: Joseph Peters
• Publication: Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31464-1. doi: 10.1016/j.cell.2020.11.005.

Backbone

• Vector backbone: pMS26
• Backbone size w/o insert (bp): 13150
• Total vector size (bp): 14555
• Vector type: Bacterial delivery vector for Tn7 att site

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Must be grown at 30C, can be cured by growth without antibiotic at 42C.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: miniTn6900 element
• Species: E. coli
• Insert Size (bp): 1337

Resource Information

• Supplemental Documents:
  • Annotated Depositor's Sequence
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector is based on pMS26 that is also available from Addgene and is described in the following reference.
Sibley, M. H., & Raleigh, E. A. (2012). A versatile element for gene addition in bacterial chromosomes. Nucleic Acids Research, 40(3), e19–e19. http://doi.org/10.1093/nar/gkr1085