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Addgene

pBS-mHopX
(Plasmid #16425)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK-
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    In Situ Template

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Hopx
  • Alt name
    Hop
  • Alt name
    Lagy
  • Alt name
    Necc1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    400
  • Mutation
    None
  • GenBank ID
    AA855657
  • Entrez Gene
    hop (a.k.a. hpy)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    IMAGE Consortium

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Antisense probes are generated by digesting with NotI and transcribing with T7 RNA Polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-mHopX was a gift from Jonathan Epstein (Addgene plasmid # 16425 ; http://n2t.net/addgene:16425 ; RRID:Addgene_16425)

  • For your References section:

    Hop is an unusual homeobox gene that modulates cardiac development. Chen F, Kook H, Milewski R, Gitler AD, Lu MM, Li J, Nazarian R, Schnepp R, Jen K, Biben C, Runke G, Mackay JP, Novotny J, Schwartz RJ, Harvey RP, Mullins MC, Epstein JA. Cell. 2002 Sep 20. 110(6):713-23. 10.1016/S0092-8674(02)00932-7 PubMed 12297045
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