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Addgene

pBS-mPlexinD1
(Plasmid #16424)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16424 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK-
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    In Situ Template

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Plexin D1
  • Alt name
    Plxnd1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Mutation
    None
  • GenBank ID
    AA138454
  • Entrez Gene
    Plxnd1 (a.k.a. 6230425C21Rik, b2b1863Clo, b2b553Clo)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Antisense probes are generated by digesting with EcoR1 and transcribing with T7 RNA Polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-mPlexinD1 was a gift from Jonathan Epstein (Addgene plasmid # 16424 ; http://n2t.net/addgene:16424 ; RRID:Addgene_16424)

  • For your References section:

    PlexinD1 and semaphorin signaling are required in endothelial cells for cardiovascular development. Gitler AD, Lu MM, Epstein JA. Dev Cell. 2004 Jul . 7(1):107-16. 10.1016/j.devcel.2004.06.002 PubMed 15239958
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