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pQCXIZ-turq-3xFLAG-TREX1 D18N
(Plasmid #164233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIZ
  • Backbone size w/o insert (bp) 7451
  • Total vector size (bp) 8393
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TREX1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    942
  • Mutation
    D18N ->nuclease deficient
  • GenBank ID
    NM_033629.6
  • Entrez Gene
    TREX1 (a.k.a. AGS1, CRV, DRN3, HERNS, RVCLS)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mturquoise (N terminal on backbone)
    • FLAG (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIZ-turq-3xFLAG-TREX1 D18N was a gift from John Maciejowski (Addgene plasmid # 164233 ; http://n2t.net/addgene:164233 ; RRID:Addgene_164233)

  • For your References section:

    ER-directed TREX1 limits cGAS activation at micronuclei. Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J. Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 10.1016/j.molcel.2020.12.037. 10.1016/j.molcel.2020.12.037 PubMed 33476576
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