RBD-Fc
(Plasmid #164221)

• Purpose: Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter
• Depositing Lab: Sriram Neelamegham
• Publication: Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 10.7554/eLife.61552.

Backbone

• Vector backbone: pCSCG
• Backbone manufacturer: Addgene plasmids 12154
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Spike RBD
• Species: SARS-CoV
• Insert Size (bp): 669
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: GCAGAGCTGGTTTAGTGAA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Haisheng Yu (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences Peking Union Medical College) and Addgene plasmid 12154

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry