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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16422 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTriEX
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5600
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Vector typeMammalian Expression
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Selectable markersPuromycin, Blasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameloxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP
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Insert Size (bp)6400
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
betaRBPIG was a gift from Juergen Hescheler (Addgene plasmid # 16422 ; http://n2t.net/addgene:16422 ; RRID:Addgene_16422) -
For your References section:
Generation of a double-fluorescent double-selectable Cre/loxP indicator vector for monitoring of intracellular recombination events. Pfannkuche K, Wunderlich FT, Doss MX, Spitkovsky D, Reppel M, Sachinidis A, Hescheler J. Nat Protoc. 2008. 3(9):1510-26. doi: https://doi.org/10.1038/nprot.2008.143 10.1038/nprot.2008.143 PubMed 18772879
Map uploaded by the depositor.
