pLEX-EF1a ANXA11-mEmerald
(Plasmid
#164210)
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PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164210 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameANXA11
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SpeciesH. sapiens (human)
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Insert Size (bp)1515
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Entrez GeneANXA11 (a.k.a. ALS23, ANX11, CAP-50, CAP50, IBMWMA)
- Promoter EF1a
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Tag
/ Fusion Protein
- mEmerald (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EF1a F
- 3′ sequencing primer WPRE R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX-EF1a ANXA11-mEmerald was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 164210 ; http://n2t.net/addgene:164210 ; RRID:Addgene_164210) -
For your References section:
RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, Using Annexin A11 as a Molecular Tether. Liao YC, Fernandopulle MS, Wang G, Choi H, Hao L, Drerup CM, Patel R, Qamar S, Nixon-Abell J, Shen Y, Meadows W, Vendruscolo M, Knowles TPJ, Nelson M, Czekalska MA, Musteikyte G, Gachechiladze MA, Stephens CA, Pasolli HA, Forrest LR, St George-Hyslop P, Lippincott-Schwartz J, Ward ME. Cell. 2019 Sep 19;179(1):147-164.e20. doi: 10.1016/j.cell.2019.08.050. 10.1016/j.cell.2019.08.050 PubMed 31539493