pCMV-MfBulKRS (human-opti)
(Plasmid
#164196)
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PurposeTo express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164196 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
- Backbone size w/o insert (bp) 5988
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMf BulKRS(human-opti)
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SpeciesMethanosarcina flavescens
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Insert Size (bp)1251
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MutationY269A;Y347F
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-forward
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The insert gene-Mf BulKRS gene ID is 53688242. The genetic codon of the insert gene has been human optimized
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-MfBulKRS (human-opti) was a gift from Tao Liu (Addgene plasmid # 164196 ; http://n2t.net/addgene:164196 ; RRID:Addgene_164196) -
For your References section:
Thermophilic Pyrrolysyl-tRNA Synthetase Mutants for Enhanced Mammalian Genetic Code Expansion. Hu L, Qin X, Huang Y, Cao W, Wang C, Wang Y, Ling X, Chen H, Wu D, Lin Y, Liu T. ACS Synth Biol. 2020 Oct 16;9(10):2723-2736. doi: 10.1021/acssynbio.0c00257. Epub 2020 Sep 23. 10.1021/acssynbio.0c00257 PubMed 32931698