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pCMV-MtAcKRS (human-opti)
(Plasmid #164195)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164195 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 5988
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MtAcKRS-478(human-opti)
  • Species
    Methanosarcina thermophila
  • Insert Size (bp)
    1434
  • Mutation
    D76G; L325M; L329I; Y330F; L333A; C372F
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

the insert gene-MtPylRS was retrieved from the uniprot database, where its ID is Q1L6A3. The genetic codon of the insert gene has been human optimized

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-MtAcKRS (human-opti) was a gift from Tao Liu (Addgene plasmid # 164195 ; http://n2t.net/addgene:164195 ; RRID:Addgene_164195)

  • For your References section:

    Thermophilic Pyrrolysyl-tRNA Synthetase Mutants for Enhanced Mammalian Genetic Code Expansion. Hu L, Qin X, Huang Y, Cao W, Wang C, Wang Y, Ling X, Chen H, Wu D, Lin Y, Liu T. ACS Synth Biol. 2020 Oct 16;9(10):2723-2736. doi: 10.1021/acssynbio.0c00257. Epub 2020 Sep 23. 10.1021/acssynbio.0c00257 PubMed 32931698
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