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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16418 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAC108L
- Backbone size w/o insert (bp) 6600
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Vector typebacmid
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameE. coli rpsL gene; tetA(C) gene
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAC-RT was a gift from Craig Strathdee (Addgene plasmid # 16418 ; http://n2t.net/addgene:16418 ; RRID:Addgene_16418) -
For your References section:
Synergy between tetA and rpsL provides high-stringency positive and negative selection in bacterial artificial chromosome vectors. Stavropoulos TA, Strathdee CA. Genomics. 2001 Feb 15. 72(1):99-104. 10.1006/geno.2000.6481 PubMed 11247671
Map uploaded by the depositor.
