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Addgene

pCAG-mEGFP-HO-G
(Plasmid #164163)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164163 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 708
  • Total vector size (bp) 4915
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mEGFP A236K
  • Species
    Synthetic
  • GenBank ID
    LC384836
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTGCTGTCTCATCATTTTGGCAAA
  • 3′ sequencing primer GATAGGCAGCCTGCACCTGAGGAGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-mEGFP-HO-G was a gift from Isei Tanida (Addgene plasmid # 164163 ; http://n2t.net/addgene:164163 ; RRID:Addgene_164163)

  • For your References section:

    Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins. Tanida I, Furuta Y, Yamaguchi J, Kakuta S, Oliva Trejo JA, Uchiyama Y. Sci Rep. 2020 Dec 14;10(1):21871. doi: 10.1038/s41598-020-78879-x. 10.1038/s41598-020-78879-x PubMed 33318540
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