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Addgene

AByI
(Plasmid #164153)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164153 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piggybac
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 9327

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    5232

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AByI was a gift from Omar Akbari (Addgene plasmid # 164153 ; http://n2t.net/addgene:164153 ; RRID:Addgene_164153)

  • For your References section:

    Site-specific transgenesis of the D. melanogaster Y-chromosome using CRISPR/Cas9. Buchman A, Akbari OS. Insect Mol Biol. 2018 Aug 6. doi: 10.1111/imb.12528. 10.1111/imb.12528 PubMed 30079589
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