AByC
(Plasmid
#164149)
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PurposeIntegrates marker gene onto D. melanogaster Y chromosome
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepiggybac
- Backbone size w/o insert (bp) 4095
- Total vector size (bp) 8963
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameY-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
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SpeciesD. melanogaster (fly)
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Insert Size (bp)4868
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AByC was a gift from Omar Akbari (Addgene plasmid # 164149 ; http://n2t.net/addgene:164149 ; RRID:Addgene_164149) -
For your References section:
Site-specific transgenesis of the D. melanogaster Y-chromosome using CRISPR/Cas9. Buchman A, Akbari OS. Insect Mol Biol. 2018 Aug 6. doi: 10.1111/imb.12528. 10.1111/imb.12528 PubMed 30079589