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Addgene

pCW57.1 CCNE1
(Plasmid #164144)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164144 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW57.1
  • Backbone size w/o insert (bp) 9354
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CCNE1
  • Alt name
    Cyclin E1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1233
  • GenBank ID
    NM_001238.4
  • Entrez Gene
    CCNE1 (a.k.a. CCNE, pCCNE1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer GAACGGACGTGAAGAATGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57.1 CCNE1 was a gift from Richard Possemato (Addgene plasmid # 164144 ; http://n2t.net/addgene:164144 ; RRID:Addgene_164144)
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