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pAAV-hGfap-R-CaMP1.07-WPRE-SV40
(Plasmid #164140)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164140 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-hGFAP-*-WPRE-SV40
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    R-CaMP1.07
  • Species
    Synthetic
  • Insert Size (bp)
    1461
  • Promoter hGFAP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Prof Junichi Nakai (Tohoku University, Japan) who has constructed R-CaMP1.07.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: The 5’ ITR and cPPT/CTS has been removed from unknown reasons. However, high-titer AAV could be derived using this plasmid in the depositing lab.

This plasmid was made by Dr. Ayumu Konno at Gunma University (Hirai Lab) via collaboration.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hGfap-R-CaMP1.07-WPRE-SV40 was a gift from Hirokazu Hirai & Hajime Hirase (Addgene plasmid # 164140 ; http://n2t.net/addgene:164140 ; RRID:Addgene_164140)

  • For your References section:

    Distinct temporal integration of noradrenaline signaling by astrocytic second messengers during vigilance. Oe Y, Wang X, Patriarchi T, Konno A, Ozawa K, Yahagi K, Hirai H, Tsuboi T, Kitaguchi T, Tian L, McHugh TJ, Hirase H. Nat Commun. 2020 Jan 24;11(1):471. doi: 10.1038/s41467-020-14378-x. 10.1038/s41467-020-14378-x PubMed 31980655
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