pSB219
(Plasmid
#164125)
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Purposep15A backbone with PhlF binding sites
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164125 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep15A-Amp
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesfGFP
- Promoter J23105
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB219 was a gift from Christopher Voigt (Addgene plasmid # 164125 ; http://n2t.net/addgene:164125 ; RRID:Addgene_164125) -
For your References section:
Single-cell measurement of plasmid copy number and promoter activity. Shao B, Rammohan J, Anderson DA, Alperovich N, Ross D, Voigt CA. Nat Commun. 2021 Mar 5;12(1):1475. doi: 10.1038/s41467-021-21734-y. 10.1038/s41467-021-21734-y PubMed 33674569
