pSB207
(Plasmid
#164122)
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PurposeJ23101 driving PP7 repeats
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 164122 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep15A-Kan
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameYFP-PP7
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SpeciesSynthetic
- Promoter J23101
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB207 was a gift from Christopher Voigt (Addgene plasmid # 164122 ; http://n2t.net/addgene:164122 ; RRID:Addgene_164122) -
For your References section:
Single-cell measurement of plasmid copy number and promoter activity. Shao B, Rammohan J, Anderson DA, Alperovich N, Ross D, Voigt CA. Nat Commun. 2021 Mar 5;12(1):1475. doi: 10.1038/s41467-021-21734-y. 10.1038/s41467-021-21734-y PubMed 33674569