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Addgene

pAAV-Neo-PTEN
(Plasmid #16411)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16411 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-MCS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression, AAV ; adeno-associated viral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PTEN Neo targeting
  • Alt name
    PTEN
  • Species
    H. sapiens (human)
  • Entrez Gene
    PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This targeting vector yields 1-5% homologous recombinants in HCT116 and other cell lines. See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Neo-PTEN was a gift from Bert Vogelstein (Addgene plasmid # 16411 ; http://n2t.net/addgene:16411 ; RRID:Addgene_16411)

  • For your References section:

    Facile methods for generating human somatic cell gene knockouts using recombinant adeno-associated viruses. Kohli M, Rago C, Lengauer C, Kinzler KW, Vogelstein B. Nucleic Acids Res. 2004 . 32(1):e3. 10.1093/nar/gnh009 PubMed 14704360
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