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PurposeDetect the short Mitochondria-Endoplasmic reticulum contact
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164108 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSplit GFP Mt-ER Short P2A
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SPLICS Mt-ER Short P2A was a gift from Marisa Brini & Tito Calì (Addgene plasmid # 164108 ; http://n2t.net/addgene:164108 ; RRID:Addgene_164108) -
For your References section:
An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo. Vallese F, Catoni C, Cieri D, Barazzuol L, Ramirez O, Calore V, Bonora M, Giamogante F, Pinton P, Brini M, Cali T. Nat Commun. 2020 Nov 27;11(1):6069. doi: 10.1038/s41467-020-19892-6. 10.1038/s41467-020-19892-6 PubMed 33247103