pAAV-Hyg-FHIT
(Plasmid
#16410)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-MCS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression, AAV ; adeno-associated viral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFHIT Hyg targeting
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Alt nameFHIT
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SpeciesH. sapiens (human)
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Entrez GeneFHIT (a.k.a. AP3Aase, FRA3B)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pAAV-Hyg-FHIT is used to generate a 1165 bp deletion of FHIT that includes exon 8 and surrounding intronic sequences.
See author's map and article for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-Hyg-FHIT was a gift from Bert Vogelstein (Addgene plasmid # 16410 ; http://n2t.net/addgene:16410 ; RRID:Addgene_16410) -
For your References section:
Facile methods for generating human somatic cell gene knockouts using recombinant adeno-associated viruses. Kohli M, Rago C, Lengauer C, Kinzler KW, Vogelstein B. Nucleic Acids Res. 2004 . 32(1):e3. 10.1093/nar/gnh009 PubMed 14704360
Map uploaded by the depositor.