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Addgene

FH1-PNGT#1-mCherry
(Plasmid #164098)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164098 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Backbone manufacturer
    David Baltimore (Addgene plasmid # 14883)
  • Backbone size w/o insert (bp) 7956
  • Vector type
    Lentiviral, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PNGT#1-mCherry
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1882

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (unknown if destroyed)
  • 3′ cloning site BsrGI (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

From TCGA datasets, we annotated the genes specific to proneural glioblastoma-subtype and identified corresponding cis-regulatory elements to generate synthetic reporters to trace subtype identity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FH1-PNGT#1-mCherry was a gift from Gaetano Gargiulo (Addgene plasmid # 164098 ; http://n2t.net/addgene:164098 ; RRID:Addgene_164098)

  • For your References section:

    Phenotypic Mapping of Pathologic Cross-Talk between Glioblastoma and Innate Immune Cells by Synthetic Genetic Tracing. Schmitt MJ, Company C, Dramaretska Y, Barozzi I, Gohrig A, Kertalli S, Grossmann M, Naumann H, Sanchez-Bailon MP, Hulsman D, Glass R, Squatrito M, Serresi M, Gargiulo G. Cancer Discov. 2021 Mar;11(3):754-777. doi: 10.1158/2159-8290.CD-20-0219. Epub 2020 Dec 23. 10.1158/2159-8290.CD-20-0219 PubMed 33361384
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