pCAG-MCS-mRuby2
(Plasmid
#164093)
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Purposeexpression of mRuby2-tagged proteins under CAG promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164093 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
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Backbone manufacturerConnie Cepko (Addgene plasmid # 11151)
- Backbone size w/o insert (bp) 4877
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRuby2
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SpeciesOther
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Insert Size (bp)729
- Promoter CAG
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Tag
/ Fusion Protein
- mRuby2 (C terminal on backbone)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byInsert derived from a plasmid from Michael Davidson (Addgene plasmid # 54614)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The original multiple cloning site was replaced by an extended MCS to improve usability of the plasmid for restriction cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-MCS-mRuby2 was a gift from Maik Hintze (Addgene plasmid # 164093 ; http://n2t.net/addgene:164093 ; RRID:Addgene_164093)